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human syndecan 4 apc conjugated antibody  (R&D Systems)


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    R&D Systems human syndecan 4 apc conjugated antibody
    Human Syndecan 4 Apc Conjugated Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+syndecan+4+apc+conjugated+antibody/Human+Syndecan-4+APC-conjugated+Antibody/pm41926337-87-4-8
    Average 94 stars, based on 9 article reviews
    human syndecan 4 apc conjugated antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Development of Anti‐Inflammatory Extracellular Vesicles by Surface Expression of Syndecan‐4
    Article Snippet: .. After a 15‐min incubation, Human Syndecan‐4 APC‐conjugated Antibody (R&D Systems) and Rat IgG2A Isotype Control (R&D Systems) were added for 30 min. .. Cells were washed, resuspended in FACS buffer, and 10,000 events per sample were collected and analyzed using FlowJo software (Tree Star Inc, Ashland, OR, USA).

    Article Title: Development of Anti-Inflammatory Extracellular Vesicles by Surface Expression of Syndecan-4
    Article Snippet: .. After a 15-minute incubation, Human Syndecan-4 APC-conjugated Antibody (R&D Systems) and Rat IgG2A Isotype Control (R&D Systems) were added for 30 minutes. .. Cells were washed, resuspended in FACS buffer, and 10,000 events per sample were collected and analyzed using FlowJo software (Tree Star Inc, Ashland, OR, USA).

    Article Title: Development of Anti-Inflammatory Extracellular Vesicles by Surface Expression of Syndecan-4.
    Article Snippet: .. After a 15-min incubation, Human Syndecan-4 APC-conjugated Antibody (R&D Systems) and Rat IgG2A Isotype Control (R&D Systems) were added for 30 min. .. Cells were washed, resuspended in FACS buffer, and 10,000 events per sample were collected and analyzed using FlowJo software (Tree Star Inc, Ashland, OR, USA).

    Control:

    Article Title: Development of Anti‐Inflammatory Extracellular Vesicles by Surface Expression of Syndecan‐4
    Article Snippet: .. After a 15‐min incubation, Human Syndecan‐4 APC‐conjugated Antibody (R&D Systems) and Rat IgG2A Isotype Control (R&D Systems) were added for 30 min. .. Cells were washed, resuspended in FACS buffer, and 10,000 events per sample were collected and analyzed using FlowJo software (Tree Star Inc, Ashland, OR, USA).

    Article Title: Development of Anti-Inflammatory Extracellular Vesicles by Surface Expression of Syndecan-4
    Article Snippet: .. After a 15-minute incubation, Human Syndecan-4 APC-conjugated Antibody (R&D Systems) and Rat IgG2A Isotype Control (R&D Systems) were added for 30 minutes. .. Cells were washed, resuspended in FACS buffer, and 10,000 events per sample were collected and analyzed using FlowJo software (Tree Star Inc, Ashland, OR, USA).

    Article Title: Development of Anti-Inflammatory Extracellular Vesicles by Surface Expression of Syndecan-4.
    Article Snippet: .. After a 15-min incubation, Human Syndecan-4 APC-conjugated Antibody (R&D Systems) and Rat IgG2A Isotype Control (R&D Systems) were added for 30 min. .. Cells were washed, resuspended in FACS buffer, and 10,000 events per sample were collected and analyzed using FlowJo software (Tree Star Inc, Ashland, OR, USA).



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    Figure 4. Effect of <t>SDC4</t> knockdown (KD) or heparin inhibition on virus internalization into Calu-3 cells. SDC4 KD in Calu-3 cells was performed previously using a lentiviral vector specific to human SDC4. (A) SDC4 expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images. Detected SDC4 levels of KD cells were normalized to WT Calu-3 cells as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05. (B,C) SDC4 KD and WT Calu-3 cells were exposed to 1 MOI of the heat-inactivated WT SCV2, Delta, and Omicron variants. For GAG inhibition, the viruses were preincubated with heparin (200 ug/mL for 30 min at 37 ◦C) before being added to the cells. (D,E) Representative flow cytometry histograms and cellular images show the intracellular fluorescence of WT or SDC4 KD Calu-3 cells treated with the viruses in the presence or absence of heparin. Scale bar = 20 µm. (F,G) Detected intracellular fluorescent signals were normalized to WT Calu-3 (F) cells or cells untreated with heparin (G) as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001.
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    Figure 4. Effect of <t>SDC4</t> knockdown (KD) or heparin inhibition on virus internalization into Calu-3 cells. SDC4 KD in Calu-3 cells was performed previously using a lentiviral vector specific to human SDC4. (A) SDC4 expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images. Detected SDC4 levels of KD cells were normalized to WT Calu-3 cells as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05. (B,C) SDC4 KD and WT Calu-3 cells were exposed to 1 MOI of the heat-inactivated WT SCV2, Delta, and Omicron variants. For GAG inhibition, the viruses were preincubated with heparin (200 ug/mL for 30 min at 37 ◦C) before being added to the cells. (D,E) Representative flow cytometry histograms and cellular images show the intracellular fluorescence of WT or SDC4 KD Calu-3 cells treated with the viruses in the presence or absence of heparin. Scale bar = 20 µm. (F,G) Detected intracellular fluorescent signals were normalized to WT Calu-3 (F) cells or cells untreated with heparin (G) as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001.
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    Image Search Results


    Figure 4. Effect of SDC4 knockdown (KD) or heparin inhibition on virus internalization into Calu-3 cells. SDC4 KD in Calu-3 cells was performed previously using a lentiviral vector specific to human SDC4. (A) SDC4 expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images. Detected SDC4 levels of KD cells were normalized to WT Calu-3 cells as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05. (B,C) SDC4 KD and WT Calu-3 cells were exposed to 1 MOI of the heat-inactivated WT SCV2, Delta, and Omicron variants. For GAG inhibition, the viruses were preincubated with heparin (200 ug/mL for 30 min at 37 ◦C) before being added to the cells. (D,E) Representative flow cytometry histograms and cellular images show the intracellular fluorescence of WT or SDC4 KD Calu-3 cells treated with the viruses in the presence or absence of heparin. Scale bar = 20 µm. (F,G) Detected intracellular fluorescent signals were normalized to WT Calu-3 (F) cells or cells untreated with heparin (G) as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001.

    Journal: International journal of molecular sciences

    Article Title: Exploring the Syndecan-Mediated Cellular Internalization of the SARS-CoV-2 Omicron Variant.

    doi: 10.3390/ijms241814140

    Figure Lengend Snippet: Figure 4. Effect of SDC4 knockdown (KD) or heparin inhibition on virus internalization into Calu-3 cells. SDC4 KD in Calu-3 cells was performed previously using a lentiviral vector specific to human SDC4. (A) SDC4 expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images. Detected SDC4 levels of KD cells were normalized to WT Calu-3 cells as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05. (B,C) SDC4 KD and WT Calu-3 cells were exposed to 1 MOI of the heat-inactivated WT SCV2, Delta, and Omicron variants. For GAG inhibition, the viruses were preincubated with heparin (200 ug/mL for 30 min at 37 ◦C) before being added to the cells. (D,E) Representative flow cytometry histograms and cellular images show the intracellular fluorescence of WT or SDC4 KD Calu-3 cells treated with the viruses in the presence or absence of heparin. Scale bar = 20 µm. (F,G) Detected intracellular fluorescent signals were normalized to WT Calu-3 (F) cells or cells untreated with heparin (G) as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001.

    Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis FlowSight, Luminex Corporation, Austin, TX, USA) with APCconjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A).

    Techniques: Knockdown, Inhibition, Virus, Plasmid Preparation, Expressing, Imaging, Cytometry

    Figure 5. SDC4 binding of WT SCV2 and the Delta an Omicron variants. (A) SDS-PAGE showing SDC4 immunoprecipitated with an antibody specific for the spike’s amino acid sequence 1000-1200 from extracts of virus-treated Calu-3 cells. Lane 1: a total of 1 ug of recombinant SDC4; lanes 2–4: immunoprecipitates of Calu-3 cells treated with either WT SCV2, Delta, or Omicron, respectively; Lane 5: immunoprecipitate of untreated control Calu-3 cells. Standard protein size markers are indicated on the right. SDC4 signals were detected with UVITEC Alliance Q9 Advanced Imager, and the intensity of bands was analyzed with the NineAlliance© software. (B) Detected band intensities were normalized to WT SCV2-treated Calu-3 cells as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; ns: not significant.

    Journal: International journal of molecular sciences

    Article Title: Exploring the Syndecan-Mediated Cellular Internalization of the SARS-CoV-2 Omicron Variant.

    doi: 10.3390/ijms241814140

    Figure Lengend Snippet: Figure 5. SDC4 binding of WT SCV2 and the Delta an Omicron variants. (A) SDS-PAGE showing SDC4 immunoprecipitated with an antibody specific for the spike’s amino acid sequence 1000-1200 from extracts of virus-treated Calu-3 cells. Lane 1: a total of 1 ug of recombinant SDC4; lanes 2–4: immunoprecipitates of Calu-3 cells treated with either WT SCV2, Delta, or Omicron, respectively; Lane 5: immunoprecipitate of untreated control Calu-3 cells. Standard protein size markers are indicated on the right. SDC4 signals were detected with UVITEC Alliance Q9 Advanced Imager, and the intensity of bands was analyzed with the NineAlliance© software. (B) Detected band intensities were normalized to WT SCV2-treated Calu-3 cells as standards. The bars represent the mean + SEM of four independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; ns: not significant.

    Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis FlowSight, Luminex Corporation, Austin, TX, USA) with APCconjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A).

    Techniques: Binding Assay, SDS Page, Immunoprecipitation, Sequencing, Virus, Recombinant, Control, Software

    Figure 6. Effect of SDC4 KD on the cellular entry and gene delivery of WT SCV2, Delta, or Omicron PSVs in Calu-3 cells. SDC4 KD and WT Calu-3 cells were treated with either WT SCV2, Delta, or Omicron PSVs. (A,B) Representative cellular images and flow cytometry histograms showing the intracellular fluorescence of WT or SDC4 KD Calu-3 cells treated with the PSVs. Scale bar = 20 µm. (C) Detected fluorescence intensities were normalized to WT Calu-3 cells treated with the respective PSVs. The bars represent the mean + SEM of three independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001.

    Journal: International journal of molecular sciences

    Article Title: Exploring the Syndecan-Mediated Cellular Internalization of the SARS-CoV-2 Omicron Variant.

    doi: 10.3390/ijms241814140

    Figure Lengend Snippet: Figure 6. Effect of SDC4 KD on the cellular entry and gene delivery of WT SCV2, Delta, or Omicron PSVs in Calu-3 cells. SDC4 KD and WT Calu-3 cells were treated with either WT SCV2, Delta, or Omicron PSVs. (A,B) Representative cellular images and flow cytometry histograms showing the intracellular fluorescence of WT or SDC4 KD Calu-3 cells treated with the PSVs. Scale bar = 20 µm. (C) Detected fluorescence intensities were normalized to WT Calu-3 cells treated with the respective PSVs. The bars represent the mean + SEM of three independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001.

    Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis FlowSight, Luminex Corporation, Austin, TX, USA) with APCconjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A).

    Techniques: Cytometry